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APPLICATION NOTE: SUPERCLEAR%u00ae TUBE REUSEPRODUCT INFORMATION VALIDATION FOR REUSELabcon%u00ae SuperClear%u00ae Tubes, engineered to prevent breakage and leakage, ensure reliable performance during strenuous processes. They are reusable, which has been vetted through rigorous testing following standard usage protocols.Effective removal of contaminants is critical for the sensitivity of molecular biology, biochemistry, and cell culture experiments. Contamination-free environments ensure integrity and prevent interference in sensitive applications like PCR and sequencing.Labcon%u2019s claims, substantiated by detailed testing, confirm the tubes meet Labcon%u2019s stringent specifications.SuperClear%u00ae Tubes have been validated for a minimum reuse of five times. They successfully passed Labcon%u2019s specifications for standard physical/functional and biological contamination testing.*Labcon%u2019s comprehensive validation and testing protocol evaluates the tubes%u2019 integrity, stability, and the efficacy of contaminant removal. The methodology involved a cyclic process of intentional contamination, washing, autoclaving, and freeze-thawing of tubes at specified intervals. Decontamination%u2019s efficacy was assessed using our standard bioassay protocols to detect residual ATP, RNase, DNase, and endotoxins. Functional integrity tests included evaluations of cap fit, leak resistance, centrifugation stability, wall thickness uniformity, gate inspection, and compliance with the 95kPa pressure test.The Earth Friendly%u00ae CompanyENVIRONMENTAL IMPACTReusing these tubes is a highly effective method for reducing the environmental footprint and costs associated with daily laboratory practices, if reuse aligns with the respective workflows and applications.After use, any claims regarding the tubes%u2019 biological state and sterility are nullified. Users must assess reuse suitablility for specific protocols. Our validation used a standard laboratory detergent effective in general cleaning, but results were inconclusive for endotoxin removal. Discarding the contents was as effective as using the detergent for this purpose, so caution is advised if endotoxin contamination is a concern.* Note on Reusability: The reusability of these tubes extends beyond the specific tests or reagents outlined in this protocol. It also is not limited by the number of cycles before tube failure.DISCLAIMER REGARDING CONTAMINATIONPHYSICAL AND CONTAMINATION TESTINGDECONTAMINATION VALIDATIONSTRESS VALIDATION